Marshall J M, Fain J N
Eur J Pharmacol. 1984 Dec 15;107(1):25-34. doi: 10.1016/0014-2999(84)90087-6.
The effects of forskolin and isoproterenol on total cyclic AMP accumulation and relaxation were compared in uterine segments from estrogen-treated rabbits and term-pregnant rats. In the rabbit, forskolin (0.2-5.0 microM) inhibited spontaneous and acetylcholine-induced contractions and increased cyclic AMP levels. At lower doses forskolin significantly inhibited contractions but had little effect on cyclic AMP (cAMP) levels. Isoproterenol (0.005-0.5 microM), doses equi-effective to those of forskolin for inhibition of contractions, did not alter cyclic AMP levels. In the rat, forskolin (0.5 microM) and isoproterenol (0.05 microM) inhibited spontaneous and potassium-induced contractions by 80%. This reduction was accompanied by an increase in cyclic AMP levels, but the increase was significantly greater with forskolin than with isoproterenol. We conclude that: (1) cAMP may mediate relaxation under certain conditions but it is not an obligatory mediator, (2) some form of compartmentalization of cyclic AMP might exist in the myometrium.
在雌激素处理过的家兔子宫段和足月妊娠大鼠子宫段中，比较了福斯高林和异丙肾上腺素对总环磷酸腺苷（cAMP）积累及舒张的影响。在家兔中，福斯高林（0.2 - 5.0微摩尔）抑制自发收缩和乙酰胆碱诱导的收缩，并增加环磷酸腺苷水平。在较低剂量时，福斯高林显著抑制收缩，但对环磷酸腺苷（cAMP）水平影响很小。异丙肾上腺素（0.005 - 0.5微摩尔），其抑制收缩的剂量与福斯高林等效，却不改变环磷酸腺苷水平。在大鼠中，福斯高林（0.5微摩尔）和异丙肾上腺素（0.05微摩尔）抑制自发收缩和钾诱导的收缩达80%。这种收缩的降低伴随着环磷酸腺苷水平的升高，但福斯高林引起的升高显著大于异丙肾上腺素。我们得出结论：（1）环磷酸腺苷在某些条件下可能介导舒张，但它不是必需的介导物；（2）子宫肌层中可能存在某种形式的环磷酸腺苷分隔。
