Rao R N
Gene. 1984 Nov;31(1-3):247-50. doi: 10.1016/0378-1119(84)90216-6.
The phage lambda N gene, contained on a HindIII-BamHI lambda DNA fragment (lambda coordinates 38 695-34 500), was inserted into plasmid pBR322 and cloned in Escherichia coli containing a defective lambda prophage. The plasmid is called pKC30. lambda N-mediated pL transcription interfered with plasmid pKC30 maintenance. Plasmid pKC30 was stabilized (i) by repression of pL, (ii) by a nutL mutation, or (iii) by cloning an "N-unresponsive terminator" sequence downstream from N gene.
位于HindIII - BamHI λ DNA片段（λ坐标38695 - 34500）上的噬菌体λ N基因被插入到质粒pBR322中，并在含有缺陷型λ原噬菌体的大肠杆菌中进行克隆。该质粒称为pKC30。λ N介导的pL转录干扰了质粒pKC30的维持。质粒pKC30通过以下方式得以稳定：（i）抑制pL，（ii）通过nutL突变，或（iii）通过在N基因下游克隆一个“N无反应终止子”序列。
