Dunn I S, Blattner F R
Nucleic Acids Res. 1987 Mar 25;15(6):2677-98. doi: 10.1093/nar/15.6.2677.
New phage lambda based cloning vectors, Charons 36-40, have been constructed which allow cloning of large (up to 24 kb) DNA fragments with up to sixteen cloning enzymes. Several of these could not be used previously with lambda vectors. Clones produced with these vectors can be propagated under recombination deficient conditions. A novel polystuffer method has been developed that permits vector arms to be purified by simple precipitation and which allows reliable identification of clones that have reincorporated any part of the stuffer. Three of the vectors are available with amber mutations in essential genes.
基于新型噬菌体λ构建了Charon 36 - 40克隆载体，这些载体可使用多达16种克隆酶克隆长达24 kb的大片段DNA。其中几种酶以前不能用于λ载体。用这些载体产生的克隆可在重组缺陷条件下繁殖。已开发出一种新的多填充片段方法，该方法允许通过简单沉淀纯化载体臂，并能可靠地鉴定重新掺入填充片段任何部分的克隆。其中三种载体在必需基因中有琥珀突变。
