卡戎36至40：具有多克隆位点和多填充片段的多酶、高容量、重组缺陷型置换载体。
Charons 36 to 40: multi enzyme, high capacity, recombination deficient replacement vectors with polylinkers and polystuffers.
作者信息
Dunn I S, Blattner F R
出版信息
Nucleic Acids Res. 1987 Mar 25;15(6):2677-98. doi: 10.1093/nar/15.6.2677.
Abstract
New phage lambda based cloning vectors, Charons 36-40, have been constructed which allow cloning of large (up to 24 kb) DNA fragments with up to sixteen cloning enzymes. Several of these could not be used previously with lambda vectors. Clones produced with these vectors can be propagated under recombination deficient conditions. A novel polystuffer method has been developed that permits vector arms to be purified by simple precipitation and which allows reliable identification of clones that have reincorporated any part of the stuffer. Three of the vectors are available with amber mutations in essential genes.
摘要
基于新型噬菌体λ构建了Charon 36 - 40克隆载体，这些载体可使用多达16种克隆酶克隆长达24 kb的大片段DNA。其中几种酶以前不能用于λ载体。用这些载体产生的克隆可在重组缺陷条件下繁殖。已开发出一种新的多填充片段方法，该方法允许通过简单沉淀纯化载体臂，并能可靠地鉴定重新掺入填充片段任何部分的克隆。其中三种载体在必需基因中有琥珀突变。
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本文引用的文献
1
Formal description of a DNA oriented computer language.一种面向DNA的计算机语言的形式化描述。
Nucleic Acids Res. 1982 Jan 11;10(1):69-84. doi: 10.1093/nar/10.1.69.
2
Purification of genomic sequences from bacteriophage libraries by recombination and selection in vivo.通过体内重组和筛选从噬菌体文库中纯化基因组序列。
Nucleic Acids Res. 1983 Apr 25;11(8):2427-45. doi: 10.1093/nar/11.8.2427.
3
Sequence of the lactose permease gene.乳糖通透酶基因序列。
Nature. 1980 Feb 7;283(5747):541-5. doi: 10.1038/283541a0.
4
Macromolecular crowding allows blunt-end ligation by DNA ligases from rat liver or Escherichia coli.大分子拥挤效应可使大鼠肝脏或大肠杆菌来源的DNA连接酶实现平端连接。
Proc Natl Acad Sci U S A. 1983 Oct;80(19):5852-6. doi: 10.1073/pnas.80.19.5852.
5
Construction of versatile expression cloning vehicles using the lipoprotein gene of Escherichia coli.利用大肠杆菌脂蛋白基因构建通用表达克隆载体。
EMBO J. 1982;1(6):771-5. doi: 10.1002/j.1460-2075.1982.tb01244.x.
6
Lambda Charon vectors (Ch32, 33, 34 and 35) adapted for DNA cloning in recombination-deficient hosts.适用于在重组缺陷宿主中进行DNA克隆的λ噬菌体Charon载体（Ch32、33、34和35）。
Gene. 1983 Dec;26(2-3):171-9. doi: 10.1016/0378-1119(83)90187-7.
7
Lambda replacement vectors carrying polylinker sequences.携带多克隆位点序列的λ置换载体。
J Mol Biol. 1983 Nov 15;170(4):827-42. doi: 10.1016/s0022-2836(83)80190-9.
8
Viability of lambda phages carrying a perfect palindrome in the absence of recombination nucleases.在缺乏重组核酸酶的情况下携带完美回文序列的λ噬菌体的生存能力。
Nature. 1983;305(5933):448-51. doi: 10.1038/305448a0.
9
New bacteriophage lambda vectors with positive selection for cloned inserts.用于对克隆插入片段进行阳性筛选的新型λ噬菌体载体。
Methods Enzymol. 1983;101:3-19. doi: 10.1016/0076-6879(83)01004-6.
10
Construction of coliphage lambda Charon vectors with BamHI cloning sites.具有BamHI克隆位点的λ噬菌体Charon载体的构建
Gene. 1980 Dec;12(3-4):301-9. doi: 10.1016/0378-1119(80)90113-4.
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