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用于在无抗生素培养基中筛选和维持携带质粒的链霉素依赖型大肠杆菌细胞的新型宿主-载体系统。

Novel host-vector system for selection and maintenance of plasmid-bearing, streptomycin-dependent Escherichia coli cells in antibiotic-free media.

作者信息

Miwa K, Nakamori S, Sano K, Momose H

出版信息

Gene. 1984 Nov;31(1-3):275-7. doi: 10.1016/0378-1119(84)90221-x.

DOI:10.1016/0378-1119(84)90221-x
PMID:6098532
Abstract

A novel system for selection and maintenance of cells carrying a recombinant plasmid has been developed, using the streptomycin-dependent (Smd) Escherichia coli 4D host and a plasmid vector carrying an rpsL gene from an Sm-resistant (Smr) mutant of E. coli which masks the Smd phenotype. Strain 4D carrying the Smr pBR322 plasmid can grow without Sm. Using this host-vector system, we can select for cells carrying an Smr recombinant plasmid and maintain them in antibiotic-free media.

摘要

利用链霉素依赖型（Smd）大肠杆菌4D宿主和携带来自大肠杆菌抗链霉素（Smr）突变体的rpsL基因的质粒载体（该基因可掩盖Smd表型），开发了一种用于选择和维持携带重组质粒细胞的新系统。携带Smr pBR322质粒的4D菌株在无链霉素的情况下能够生长。使用这种宿主-载体系统，我们可以选择携带Smr重组质粒的细胞，并将它们维持在无抗生素的培养基中。

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