Schulster D, Salmon D M
J Recept Res. 1984;4(1-6):301-13. doi: 10.3109/10799898409042557.
Isolated adrenal fasciculata cells were purified by centrifugation through a 0-50% hyperbolic gradient of PercollR. The dose-dependence and kinetics of both intracellular cyclic AMP accumulation and steroidogenesis in response to ACTH1-39 and ACTH5-24 (corticotropin-(1-39) and corticotropin-(5-24)-peptides) were determined using purified cells. The rate of intracellular cyclic AMP formation was maximal during the first five minutes after hormone addition and remained constant or fell thereafter. Therefore intracellular cyclic AMP accumulation, assessed after 5 min., was compared with steroid output after 20 min. Maximal steroidogenesis was elicited by ACTH5-24 without discerning a significant stimulation of intracellular cyclic AMP accumulation. ACTH6-24 (corticotropin-(6-24)-peptide) could completely inhibit the intracellular cyclic AMP accumulation elicited by ACTH1-39 or by ACTH5-24 at concentrations that only partially inhibited steroidogenesis. It is possible that there are two pathways for the steroidogenic action of ACTH, one of which is obligatorily mediated by intracellular cyclic AMP, and another which involves a different mediator.
通过在0-50%的PercollR双曲线梯度中离心，纯化分离的肾上腺束状带细胞。使用纯化的细胞确定细胞内环状AMP积累和类固醇生成对促肾上腺皮质激素1-39（促肾上腺皮质激素-（1-39）肽）和促肾上腺皮质激素5-24（促肾上腺皮质激素-（5-24）肽）的剂量依赖性和动力学。激素添加后的前五分钟内细胞内环状AMP形成速率最大，此后保持恒定或下降。因此，比较了5分钟后评估的细胞内环状AMP积累与20分钟后的类固醇产量。促肾上腺皮质激素5-24引发了最大的类固醇生成，而未发现对细胞内环状AMP积累有明显刺激。促肾上腺皮质激素6-24（促肾上腺皮质激素-（6-24）肽）在仅部分抑制类固醇生成的浓度下，可完全抑制促肾上腺皮质激素1-39或促肾上腺皮质激素5-24引发的细胞内环状AMP积累。促肾上腺皮质激素的类固醇生成作用可能有两条途径，其中一条必然由细胞内环状AMP介导，另一条涉及不同的介质。
