Roduit C, Pralong W F, Zahnd G R, Schlegel W
J Recept Res. 1984;4(1-6):505-20. doi: 10.3109/10799898409042570.
HPLC methodology has found wide application in analytical problems in biochemistry. To study the metabolism of phosphatidylinositol and its regulation by receptor mediated events, HPLC could be a valuable technique. It has been recently demonstrated that a variety of hormones and neurotransmittors act to stimulate hydrolysis of phosphoinositides by a phospholipase C. To monitor this reaction, we have analysed the formation of radiolabelled inositol phosphates from phosphoinositides. The present paper describes a rapid HPLC procedure, to separate inositol phosphates from myo-inositol, which could be used in pharmacological studies of receptors linked to phosphoinositide hydrolysis. The potential of the application of HPLC to the analysis of the phospholipids involved is discussed.
高效液相色谱法（HPLC）在生物化学分析问题中已得到广泛应用。为了研究磷脂酰肌醇的代谢及其受受体介导事件的调节，HPLC可能是一项有价值的技术。最近已证明，多种激素和神经递质通过磷脂酶C刺激磷酸肌醇的水解。为监测此反应，我们分析了从磷酸肌醇生成放射性标记的肌醇磷酸的情况。本文描述了一种快速的HPLC程序，用于从肌醇中分离肌醇磷酸，该程序可用于与磷酸肌醇水解相关受体的药理学研究。还讨论了HPLC在分析相关磷脂方面的应用潜力。
