Separation and collection of rat liver macrophages.
作者信息
Garvey J S, Heil M F
出版信息
Methods Enzymol. 1984;108:285-94. doi: 10.1016/s0076-6879(84)08093-9.
PMID:6098797
Abstract
摘要
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Separation and collection of rat liver macrophages.大鼠肝脏巨噬细胞的分离与收集
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Preparation of pure hepatocytes and reticuloendothelial cells in high yield from a single rat liver by means of Percoll centrifugation and selective adherence.通过Percoll离心和选择性贴壁从单只大鼠肝脏中高产率制备纯肝细胞和网状内皮细胞。
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Use of Percoll density gradient centrifugation for preparing isolated rat hepatocytes having long-term viability.使用Percoll密度梯度离心法制备具有长期活力的分离大鼠肝细胞。
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Isolation and culture of hepatic lipocytes, Kupffer cells, and sinusoidal endothelial cells by density gradient centrifugation with Stractan.使用Stractan通过密度梯度离心法分离和培养肝脂肪细胞、库普弗细胞和肝血窦内皮细胞。
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