Lohse M J, Klotz K N, Ukena D, Schwabe U
Neurosci Lett. 1984 Nov 23;52(1-2):97-101. doi: 10.1016/0304-3940(84)90357-4.
The binding of [3H]phenobarbital to rat brain membranes was studied in order to determine its characteristics and specificity. The binding reaction was rapid and occurred at sites of low affinity. (Kd = 700 microM) and very high density (Bmax = 2.7 nmol/mg protein). It was unaffected by temperature changes from 0 degrees C to 95 degrees C and was maximal at pH 5. Detergents in low concentrations markedly decreased the binding, apparently without solubilizing the binding sites. It is concluded that the binding of [3H]phenobarbital is a rather non-specific interaction with the plasma membrane.
为了确定[3H]苯巴比妥与大鼠脑膜的结合特性和特异性，对其进行了研究。结合反应迅速，发生在低亲和力（解离常数Kd = 700微摩尔）和非常高密度（最大结合容量Bmax = 2.7纳摩尔/毫克蛋白质）的位点。从0摄氏度到95摄氏度的温度变化对其没有影响，在pH 5时结合量最大。低浓度的去污剂显著降低了结合，显然没有使结合位点溶解。得出的结论是，[3H]苯巴比妥的结合是与质膜的一种相当非特异性的相互作用。
