通过UTP-琼脂糖亲和层析从猫肝脏高尔基体中纯化UTP：D-葡萄糖-1-磷酸尿苷酰转移酶
Purification of a UTP:D-glucose-1-phosphate uridylyltransferase from Golgi apparatus of cat liver by affinity chromatography on UTP-agarose.
作者信息
Azzar G, Persat F, Degiuli A, Got R
出版信息
Anal Biochem. 1984 Nov 1;142(2):518-20. doi: 10.1016/0003-2697(84)90499-8.
PMID:6099063
Abstract
UDP-glucose pyrophosphorylase from Golgi apparatus solubilized by detergent has been purified 100-fold from microsomes by affinity chromatography on UTP-agarose. The purified enzyme has apparent Mr 270,000 and isoelectric pH 3.9 against 360,000 and 4.2 for soluble enzyme. According to these characteristics, UDP-glucose pyrophosphorylase from Golgi apparatus is different from cytosolic enzyme.
摘要
用去污剂溶解的来自高尔基体的UDP - 葡萄糖焦磷酸化酶，通过在UTP - 琼脂糖上的亲和层析，已从微粒体中纯化了100倍。纯化后的酶表观分子量为270,000，等电点pH为3.9，而可溶性酶的表观分子量为360,000，等电点pH为4.2。根据这些特性，来自高尔基体的UDP - 葡萄糖焦磷酸化酶与胞质酶不同。
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