氯化锂对囊胚期之后小鼠胚胎分化的影响。
Effect of LiCl on differentiation of mouse embryos beyond the blastocyst stage.
作者信息
Fernández M S, Izquierdo L
出版信息
Arch Biol Med Exp. 1983 Aug;16(1):51-4.
In order to test the effect of Li+ on cell differentiation, 2-cell embryos were cultured in vitro with or without Li+ to the blastocyst stage and then, in a "implantation" culture medium, with or without Li+, up the early egg-cylinder stage. Our results show: 1) that Li+ in the preimplantation medium diminishes "implantation" success significantly; 2) that trophoblastic vesicles, produced by culturing preimplantation embryos with Li+, do not generate an inner cell mass when further cultured in "implantation" medium; 3) that the ultrastructure of "implanted" embryos is similar in all experimental series.
为了测试锂离子对细胞分化的影响，将二细胞胚胎在含有或不含有锂离子的情况下体外培养至囊胚阶段，然后在“着床”培养基中，在含有或不含有锂离子的情况下，培养至早期卵圆柱阶段。我们的结果表明：1）着床前培养基中的锂离子显著降低“着床”成功率；2）用锂离子培养着床前胚胎产生的滋养层囊泡，在“着床”培养基中进一步培养时不会产生内细胞团；3）在所有实验系列中，“着床”胚胎的超微结构相似。
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