Studdert M J, Fitzpatrick D R, Horner G W, Westbury H A, Gleeson L J
Aust Vet J. 1984 Nov;61(11):345-8. doi: 10.1111/j.1751-0813.1984.tb07152.x.
Representative strains of EHV isolated from an aborted foetus and from a horse with rhinopneumonitis in New Zealand had restriction endonuclease DNA fingerprints typical of those usually associated with these syndromes elsewhere and now designated EHV1 and 4 respectively. EHV1 was isolated from the brain and spinal cord of a 4-year-old gelding that died of myeloencephalitis. A mare on the same farm, at about the same time as the gelding developed myeloencephalitis, aborted and EHV1 was isolated from the tissues of the aborted foetus. Restriction endonuclease DNA fingerprints of the viruses isolated from myeloencephalitis and abortion were indistinguishable by Bam HI but were distinguishable using Bgl I, Pvu II, Xho I and Hind III. The restriction endonuclease DNA fingerprints of 3 EHV1 strains known to cause myeloencephalitis were compared with each other and with EHV1 strains not known to be associated with myeloencephalitis. The Bgl I Pvu II and Hind III DNA fingerprints of the 3 myeloencephalogenic strains appear distinguishable from non-myeloencephalogenic strains. Abortion was induced in a mare by intrauterine inoculation of EHV4. The Bam HI, Bgl I, Pvu II, Xho I and Hind III restriction endonuclease DNA fingerprints of the inoculum virus were indistinguishable from virus recovered from the foetus. It was concluded that passage of the virus through the foetus did not detectably alter the restriction endonuclease DNA fingerprint.
从新西兰一匹流产胎儿和一匹患鼻肺炎的马中分离出的马疱疹病毒（EHV）代表性毒株，其限制性内切酶DNA指纹图谱具有典型特征，通常与其他地方的这些综合征相关，现在分别被命名为EHV1和EHV4。EHV1是从一匹死于脑脊髓炎的4岁阉割公马的脑和脊髓中分离出来的。在同一农场的一匹母马，大约在这匹阉割公马患脑脊髓炎的同时流产，并且从流产胎儿的组织中分离出了EHV1。从脑脊髓炎和流产病例中分离出的病毒的限制性内切酶DNA指纹图谱，用Bam HI无法区分，但用Bgl I、Pvu II、Xho I和Hind III可以区分。将已知会引起脑脊髓炎的3株EHV1毒株的限制性内切酶DNA指纹图谱相互比较，并与已知与脑脊髓炎无关的EHV1毒株进行比较。这3株致脑脊髓炎毒株的Bgl I、Pvu II和Hind III DNA指纹图谱似乎与非致脑脊髓炎毒株不同。通过向一匹母马子宫内接种EHV4诱导其流产。接种病毒的Bam HI、Bgl I、Pvu II、Xho I和Hind III限制性内切酶DNA指纹图谱与从胎儿中回收的病毒无法区分。得出的结论是，病毒通过胎儿传播并没有明显改变限制性内切酶DNA指纹图谱。
