[3H]可乐定与溶解的大鼠脑α2-肾上腺素能受体结合的初始检测：鸟嘌呤核苷酸的调节作用
Initial detection of [3H]clonidine binding to solubilized rat brain alpha 2-adrenergic receptors: regulation by guanine nucleotides.
作者信息
Matsui H, Imafuku J, Asakura M, Tsukamoto T, Ino M, Saitoh N, Miyamura S, Hasegawa K
出版信息
Brain Res. 1984 Dec 24;324(2):369-73. doi: 10.1016/0006-8993(84)90052-0.
PMID:6099206
Abstract
Alpha 2-adrenergic receptors labeled by [3H]clonidine (alpha 2-agonist) can be solubilized from the rat brain with a zwitterionic detergent, 3-[(3-cholamidopropyl)-dimethylammonio]-1-propane sulfonate (CHAPS). CHAPS-solubilized receptors have the same characteristics of membrane-bound alpha 2-receptors in the brain, and the regulation of receptor binding by guanine nucleotides is retained in the soluble state.
摘要
用[3H]可乐定（α2-激动剂）标记的α2-肾上腺素能受体可用两性离子去污剂3-[(3-胆酰胺丙基)-二甲基铵]-1-丙烷磺酸盐（CHAPS）从大鼠脑中溶解出来。CHAPS溶解的受体具有与脑中膜结合α2-受体相同的特性，并且鸟嘌呤核苷酸对受体结合的调节在溶解状态下得以保留。
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