高效液相色谱法与毛细管气相色谱法分析Δ⁹-四氢大麻酚胶囊的比较

Comparison of the analysis of delta 9-tetrahydrocannabinol capsules by high-performance liquid chromatography and capillary gas chromatography.

作者信息

Ray G, Crook M, West N, Kwoka M, Rehagen G, Cox J, Murrill E, Flora K

出版信息

J Chromatogr. 1984 Dec 28;317:455-62. doi: 10.1016/s0021-9673(01)91685-1.

Abstract

The modification of a high-performance liquid chromatography (HPLC) system and the development of a capillary gas-chromatographic (GC) system for the analysis of delta 9-tetrahydrocannabinol, encapsulated in soft gelatin capsules, are described. A photodiode array detector was used to evaluate peak homogeneity after each HPLC system modification. Sesame oil was separated from the extract by Sep-Pak filtration prior to GC analysis. Quantitation by both systems had r values greater than 0.999 and R.S.D. values less than 1.0%. Simultaneous capsule assays by both methods agreed within 1%.

摘要

描述了一种用于分析软胶囊中Δ⁹-四氢大麻酚的高效液相色谱（HPLC）系统的改进以及毛细管气相色谱（GC）系统的开发。在每次HPLC系统改进后，使用光电二极管阵列检测器评估峰的同质性。在进行GC分析之前，通过Sep-Pak过滤从提取物中分离出芝麻油。两种系统的定量分析r值均大于0.999，相对标准偏差（R.S.D.）值小于1.0%。两种方法同时进行的胶囊分析结果相差在1%以内。