Koziel M G, Adams T L, Hazlet M A, Damm D, Miller J, Dahlbeck D, Jayne S, Staskawicz B J
J Mol Appl Genet. 1984;2(6):549-62.
The promoter region of the CaMV inclusion body protein gene was modified for use in chimeric gene fusions. The modified promoter was used to construct a selectable marker for plant transformation based on the Tn 5 kanamycin resistance gene. This chimeric selectable marker was introduced into plant cells using oncogenic and deoncogenized strains of Agrobacterium tumefaciens. Both types of transformation produced kanamycin-resistant cell lines. The resistant cell lines derived from the deoncogenized strains were used to regenerate shoots. A second type of selection based on the ability of octopine synthase to detoxify aminoethyl cysteine was also used to select transformants in both oncogenic and nononcogenic transformation.
对花椰菜花叶病毒（CaMV）包涵体蛋白基因的启动子区域进行了修饰，以用于嵌合基因融合。修饰后的启动子用于构建基于Tn 5卡那霉素抗性基因的植物转化选择标记。使用致癌和去致癌的根癌农杆菌菌株将这种嵌合选择标记导入植物细胞。两种转化类型均产生了卡那霉素抗性细胞系。来自去致癌菌株的抗性细胞系用于再生芽。还基于章鱼碱合酶解毒氨基乙基半胱氨酸的能力进行了第二种选择，以在致癌和非致癌转化中选择转化体。
