Eilam Y, Lavi H, Grossowicz N
Microbios. 1984;41(165-166):177-89.
The plasma membrane ATPase inhibitors N,N-dicyclohexylcarbodiimide (DCCD), diethylstilboestrol and sodium orthovanadate caused inhibition of proton ejection in Saccharomyces cerevisiae at a concentration range of 0.1-0.4 mM. At this concentration K+ efflux was not observed; DCCD caused K+ efflux only at a much higher concentration (2mM). It was shown that induction of K+ efflux by DCCD was not mediated via inhibition of ATPase and that it may be the result of a direct effect of the drug on the cell membrane or on the K+ carrier. All three inhibitors also caused mild hyperpolarization of the membrane and an increase in the carrier mediated Ca2+ uptake into the cells. The increase in delta psi balanced the decrease in delta pH so that the value of delta mu H+ changed very little following incubation of the cells with the inhibitors. The respiratory deficient mutant ('petite') displayed similar phenomena to the wild type, but membrane potential values were lower than in the wild type.
质膜ATP酶抑制剂N，N -二环己基碳二亚胺（DCCD）、己烯雌酚和原钒酸钠在0.1 - 0.4 mM的浓度范围内可抑制酿酒酵母中的质子排出。在此浓度下未观察到钾离子外流；DCCD仅在高得多的浓度（2 mM）时才会导致钾离子外流。结果表明，DCCD诱导的钾离子外流不是通过抑制ATP酶介导的，可能是该药物对细胞膜或钾离子载体直接作用的结果。所有这三种抑制剂还会引起膜的轻度超极化以及载体介导的钙离子摄取到细胞中的增加。膜电位差（Δψ）的增加平衡了质子动力势（ΔpH）的降低，因此在用抑制剂孵育细胞后，质子电化学势（ΔμH⁺）的值变化很小。呼吸缺陷型突变体（“小菌落”）表现出与野生型相似的现象，但膜电位值低于野生型。
