增加膜多不饱和脂肪酸含量可增强NIE-115神经母细胞瘤中环磷酸腺苷的形成和前列腺素的产生。

Increasing membrane polyunsaturated fatty-acid content augments cyclic AMP formation and prostaglandin production in NIE-115 neuroblastoma.

作者信息

Murphy M G

出版信息

Prog Neuropsychopharmacol Biol Psychiatry. 1984;8(4-6):529-37. doi: 10.1016/0278-5846(84)90011-3.

Abstract

Addition of linoleic acid (50 microM) to culture medium significantly increases levels of polyunsaturated fatty acids (PUFA) in membrane phospholipids of NIE-115 neuroblastoma. Basal levels of cyclic AMP are elevated significantly in supplemented cells. Exogenous prostaglandins (PG) PGE1 and PGD2 stimulate cAMP formation in NIE-115 neuroblastoma. Supplemented cells produce higher levels of PGE and PGD than do control cultures. Inclusion of cyclooxygenase inhibitors in culture medium does not block elevation of cyclic nucleotide in supplemented cells. Endogenous PG production and receptor activation cannot account for increased cAMP in EFA-supplemented neuroblastoma.

摘要

向培养基中添加亚油酸（50微摩尔）可显著提高NIE - 115神经母细胞瘤细胞膜磷脂中多不饱和脂肪酸（PUFA）的水平。补充亚油酸的细胞中环磷酸腺苷（cAMP）的基础水平显著升高。外源性前列腺素（PG）PGE1和PGD2可刺激NIE - 115神经母细胞瘤中cAMP的形成。与对照培养物相比，补充亚油酸的细胞产生更高水平的PGE和PGD。在培养基中加入环氧化酶抑制剂并不能阻止补充亚油酸的细胞中环核苷酸的升高。内源性PG的产生和受体激活不能解释补充必需脂肪酸（EFA）的神经母细胞瘤中cAMP的增加。