Alonso-Caplen F V, Matsuoka Y, Wilcox G E, Compans R W
Virus Res. 1984;1(2):153-67. doi: 10.1016/0168-1702(84)90070-4.
Avian infectious bronchitis virus (IBV) was adapted to Vero cells by serial passage. No significant inhibition of IBV replication was observed when infected Vero cells were treated with alpha-amanitin or actinomycin D. In thin sections of infected cells, assembly of IBV was observed at the rough endoplasmic reticulum (RER), and mature IBV particles were located in dilated cisternae of the RER as well as in smooth cytoplasmic vesicles. In addition to typical IBV particles, enveloped particles containing numerous ribosomes were identified at later times postinfection. Monensin, a sodium ionophore which blocks glycoprotein transport to plasma membranes at the level of the Golgi complex, was found to inhibit the formation of infectious IBV. In thin sections of infected Vero cells treated with the ionophore, IBV particles were located in dilated cytoplasmic vesicles, but fewer particles were found when compared to controls. A similar pattern of virus-specific proteins was detected in control or monensin-treated IBV-infected cells, which included two glycoproteins (170 000 and 24 000 daltons) and a polypeptide of 52 000 daltons. These results suggest that the ionophore inhibits assembly of a virus which matures at intracellular membranes.
禽传染性支气管炎病毒（IBV）通过连续传代适应了Vero细胞。当用α-鹅膏蕈碱或放线菌素D处理感染的Vero细胞时，未观察到对IBV复制的显著抑制。在感染细胞的超薄切片中，观察到IBV在粗面内质网（RER）处组装，成熟的IBV颗粒位于RER的扩张池以及光滑的细胞质囊泡中。除了典型的IBV颗粒外，在感染后较晚时间还鉴定出含有大量核糖体的包膜颗粒。莫能菌素是一种钠离子载体，可在高尔基体水平阻断糖蛋白向质膜的转运，发现它能抑制传染性IBV的形成。在用离子载体处理的感染Vero细胞的超薄切片中，IBV颗粒位于扩张的细胞质囊泡中，但与对照相比发现的颗粒较少。在对照或莫能菌素处理的IBV感染细胞中检测到类似的病毒特异性蛋白模式，其中包括两种糖蛋白（170 000和24 000道尔顿）和一种52 000道尔顿的多肽。这些结果表明，离子载体抑制了在内膜成熟的病毒的组装。
