慢反应物质（SRSs）：大鼠嗜碱性粒细胞白血病（RBL-1）细胞中慢反应物质的结构鉴定

Slow reacting substances (SRSs): the structure identification of SRSs from rat basophil leukaemia (RBL-1) cells.

作者信息

Morris H R, Taylor G W, Piper P J, Samhoun M N, Tippins J R

出版信息

Prostaglandins. 1980 Feb;19(2):185-201. doi: 10.1016/0090-6980(80)90019-2.

Abstract

Slow Reacting Substances have been produced from RBL-l cells by calcium ionophore A23187 and purified to homogeneity by high pressure liquid chromatography (HPLC). The structure of the major biologically active species has been determined by mass spectrometric examination of the intact molecule as a derivative, together with amino-acid analysis and sequence determination. The characteristic triene chromophore which we originally identified in immunologically generated SRS-A is present in RBL-l SRS, and we determine the structure of this SRS as the thio-substituted dipeptide, 5-hydroxy-6-cysteinylglycinyl-7,9,11,14-eicosatetraenoic acid.

摘要

钙离子载体A23187已从RBL-1细胞中产生慢反应物质，并通过高压液相色谱（HPLC）纯化至同质。主要生物活性物质的结构已通过对完整分子衍生物的质谱分析、氨基酸分析和序列测定来确定。我们最初在免疫产生的SRS-A中鉴定出的特征性三烯发色团存在于RBL-1 SRS中，我们将这种SRS的结构确定为硫代取代的二肽，即5-羟基-6-半胱氨酰甘氨酰-7,9,11,14-二十碳四烯酸。