Pasini N
Ann Microbiol (Paris). 1977 Nov-Dec;128B(4):547-51.
In the embryonated 4-5 days old chicken egg incubated in the normal vertical position, only the blunt top end, a small part of the egg surface, is covered by the embryo. Soon after rotation of the egg to the reverted vertical position with the blunt end down, the egg shell is pierced and the artificial air space is formed on the pointed end which is far from the region occupied by the embryo. Thus any possibility of lesion is avoided.
在正常垂直位置孵化4 - 5天的带胚胎鸡蛋中，只有钝端顶部，即鸡蛋表面的一小部分，被胚胎覆盖。在将鸡蛋旋转至钝端向下的倒置垂直位置后不久，蛋壳被刺破，在远离胚胎所占区域的尖端形成人工气室。这样就避免了任何损伤的可能性。
