Cell-mediated synthesis of somatostatin.

Messenger RNA from catfish pancreatic islets was translated in a wheat germ cell-free protein synthesizing system. A protein of 12,000 mol wt, preprosomatostatin, was identified by specific immunoprecipitaton with anti-catfish pancreatic somatostatin and sodium dodecyl sulfate (SDS)- polyacrylamide gel electrophoresis.