Krisch B, Leonhardt H
Cell Tissue Res. 1980;211(2):251-68. doi: 10.1007/BF00236448.
With the aid of electron microscopic immunocytochemistry following the application of antisera against somatostatin and luliberin (LRF), a labeling of the intercellular clefts in different areas of the brain was observed. This labeling is especially conspicuous near the basal pose of the cuboidal ependymal cells, but is also generally present in all regions containing neurohormone-producing perikarya or their processes (for example, the preoptic area, the basal ganglia and the cortex). Furthermore, in all these regions displaying labeled intercellular clefts, glia-like cells and sparsely ciliated ependymal cells are found, the secondary lysosomes of which exhibit an immunoreactivity resembling that observed in the intercellular clefts. As sources of the immunoreactive material the following possibilities are discussed: (i) perikarya producing somatostatin or LRF, situated in the wall of the third ventricle and sending fibers between the cuboidal ependymal cells, (ii) hypothalamic and extrahypothalamic projections of both peptidergic systems, and (iii) in the case of somatostatin, immunoreactive perikarya in the cortex.
应用抗生长抑素和促黄体素释放激素（LRF）抗血清后，借助电子显微镜免疫细胞化学技术，观察到大脑不同区域细胞间缝隙的标记情况。这种标记在立方上皮细胞的基底部位附近尤为明显，但在所有含有产生神经激素的神经核周体或其突起的区域（例如视前区、基底神经节和皮质）也普遍存在。此外，在所有显示有标记的细胞间缝隙的区域，都发现了类胶质细胞和稀疏纤毛的室管膜细胞，其次级溶酶体呈现出与细胞间缝隙中观察到的免疫反应性相似的反应。作为免疫反应性物质的来源，讨论了以下几种可能性：（i）位于第三脑室壁产生生长抑素或LRF的神经核周体，并在立方上皮细胞之间发出纤维；（ii）两种肽能系统的下丘脑和下丘脑外投射；（iii）就生长抑素而言，皮质中的免疫反应性神经核周体。
