使用高效正相液相色谱法测定人血浆中的Org NC 45（一种神经肌肉阻滞剂）。

Determination of Org NC 45 (a myoneural blocking agent) in human plasma using high-performance normal-phase liquid chromatography.

作者信息

Paanakker J E, van de Laar G L

出版信息

J Chromatogr. 1980 Oct 10;183(4):459-66. doi: 10.1016/s0378-4347(00)81588-1.

Abstract

The assay for the quantification of Org NC 45 in human plasma is described in detail. It comprises ion-pair extraction and normal-phase liquid chromatography in conjunction with UV detection. An analogue, 17 beta-deacetyl-Org NC 45, is used as standard in the assay procedure. The accuracy and precision of the assay at 400 ng of Org NC 45 per ml of plasma are 0.7% and 2.8%, respectively. The detection limit is approx. 50 ng Org NC 45 per ml of plasma. The assay can be used for the pharmacokinetic evaluation of Org NC 45 in man.

摘要

本文详细描述了人血浆中Org NC 45的定量分析方法。该方法包括离子对萃取和正相液相色谱结合紫外检测。在分析过程中，使用类似物17β-去乙酰基-Org NC 45作为标准品。每毫升血浆中含有400 ng Org NC 45时，该分析方法的准确度和精密度分别为0.7%和2.8%。检测限约为每毫升血浆50 ng Org NC 45。该分析方法可用于人体中Org NC 45的药代动力学评估。