Pickford G E, Knight W R, Knight J N
Rev Can Biol. 1980 Jun;39(2):97-105.
Arginine vasotocin, injected into the third vehicle of 10 brain-cannulated, unanesthetized male Fundulus heteroclitus at estimated doses of 0.4 and 40 mU/g wt., failed to elicit a spawning reflex in five recipients; each had been both pre- and post-tested for responsiveness to intraperitoneal injections. Five others responded at the 40 mU/g dose and one also at the 0.4 mU/g dose: the first response occurred after a long delay, similar to that after intraperitoneal injection. Escape into the peripheral circulation is implicated. Since the N. preopticus is known to participate in the spawning reflex response, an indirect pathway from the peripheral AVT-receptor to the hypothalamic nucleus is suspected. Substances that might have behavioral effects, or function as neuro-transmitters, were tested with negative results: ACTH, epinephrine, norepinephrine, GABA, dopamine, L-dopa, cAMP, cGMP and the prostaglandins PGA2, PGE2 and PGE2 alpha. Serotonin failed to elicit a spawning reflex but caused rapidly developing symptoms of hypoxia. An immediate single spawning reflex was observed on three occasions after intracerebral injection: once after each of the otherwise ineffective hypothalamic releasing hormones, LRF and TRF, and once after AVP. Direct stimulation of the N. preopticus is suspected.
向10只脑插管、未麻醉的雄性底鳉注射精氨酸血管加压素，估计剂量为0.4和40 mU/g体重，注射到第三脑室，5只接受者未引发产卵反射；每只接受者在腹腔注射前和注射后都进行了反应性测试。另外5只在40 mU/g剂量时有反应，1只在0.4 mU/g剂量时也有反应：首次反应出现延迟很长时间，类似于腹腔注射后的情况。这表明精氨酸血管加压素逃逸到外周循环中。由于已知视前核参与产卵反射反应，因此怀疑存在从外周精氨酸血管加压素受体到下丘脑核的间接途径。对可能具有行为效应或作为神经递质起作用的物质进行了测试，结果均为阴性：促肾上腺皮质激素、肾上腺素、去甲肾上腺素、γ-氨基丁酸、多巴胺、左旋多巴、环磷酸腺苷、环磷酸鸟苷以及前列腺素PGA2、PGE2和PGE2α。血清素未能引发产卵反射，但导致缺氧症状迅速出现。脑内注射后有三次观察到立即出现单次产卵反射：分别在原本无效的下丘脑释放激素促黄体释放因子和促甲状腺激素释放因子注射后各出现一次，以及在血管加压素注射后出现一次。怀疑是对视前核的直接刺激。
