Voaden M J, Morjaria B
J Neurochem. 1980 Jul;35(1):95-9. doi: 10.1111/j.1471-4159.1980.tb12493.x.
The effect of light stimulation in vitro on the labelling of neuroactive amino acids derived from [14C]glucose or [14C]glutamine in the rat retina has been studied. [14C]Glutamine, at 700 microM, provided about 50% of the tissue pools of glutamate, aspartate, and GABA; and the labelling of these decreased on light stimulation, both in the photoreceptor cells (glu and asp) and in the inner retina (glu, asp, and GABA). In contrast, there were no significant changes in the entry of label derived from [14C]glucose, although similar trends were apparent in the data obtained for the photoreceptor cell layer. The pools may, therefore, be separate. Other results support the contention that glucose is the principal energy source for the retina, its entry into non-amino acid derivates being decreased on light stimulation.
研究了体外光刺激对大鼠视网膜中源自[14C]葡萄糖或[14C]谷氨酰胺的神经活性氨基酸标记的影响。700微摩尔的[14C]谷氨酰胺提供了约50%的谷氨酸、天冬氨酸和GABA的组织池；在光刺激下，这些氨基酸在光感受器细胞（谷氨酸和天冬氨酸）和视网膜内层（谷氨酸、天冬氨酸和GABA）中的标记均减少。相比之下，源自[14C]葡萄糖的标记进入量没有显著变化，尽管在光感受器细胞层获得的数据中也有类似趋势。因此，这些池可能是分开的。其他结果支持这样的观点，即葡萄糖是视网膜的主要能量来源，其进入非氨基酸衍生物的量在光刺激下减少。
