Torres J, Klein R M, Tung H N, Chapman A L
Virchows Arch B Cell Pathol Incl Mol Pathol. 1980;33(2):139-53. doi: 10.1007/BF02899177.
The cell cycle kinetics of uninfected and feline leukemia virus-infected canine lymphoma cell lines were determined by autoradiography (PLM method) as follows: DT-5: generation time (TC), 15.2 h; pre-synthetic gap phase (TG1), 3.2 h; DNA-synthetic phase (TS), 8.2 h; post-synthetic gap ph se (TG2), 3.3 h; visible mitotic phase (TM), 0.5 h. 11028: TC, 13.6 h; TG1, 1.9 h; TS, 7.7 h; TG2, 3.4 h; TM, 0.6 h. 11028+FeLV (11028 productively infected with feline leukemia virus): TC, 11.2 h; TG1, 0.2 h; TS, 8.3 h; TG2, 2.1 h; TM, 0.6 h. Exposure of the lymphoma cell lines to methotrexate (MTX) in vitro produces dose-related increases in cellular volume, associated with reductions in cellular proliferation. The relative sensitivities of these cell lines to MTX, measured by the ID50 MTX concentrations for DT-5, 11028, and 11028+FeLV are 118 nM, 122 nM, and 28 nM respectively. The cell kinetic effects of the ID50 MTX concentrations added to cultures of lymphoma cells pulse-labeled with tritiated thymidine are an approximately 2-h prolongation of TC, attributable to a lengthening of TS, with other cell cycle phases not significantly altered. These cell lines are highly tumorigenic when transplanted into the cheek pouches of immunosuppressed hamsters, with inocula of 10(4) cells producing rapidly growing, well vascularized tumors.
通过放射自显影术（PLM法）测定未感染和感染猫白血病病毒的犬淋巴瘤细胞系的细胞周期动力学如下：DT-5：倍增时间（TC），15.2小时；合成前期（TG1），3.2小时；DNA合成期（TS），8.2小时；合成后期（TG2），3.3小时；可见有丝分裂期（TM），0.5小时。11028：TC，13.6小时；TG1，1.9小时；TS，7.7小时；TG2，3.4小时；TM，0.6小时。11028 + FeLV（11028被猫白血病病毒有效感染）：TC，11.2小时；TG1，0.2小时；TS，8.3小时；TG2，2.1小时；TM，0.6小时。淋巴瘤细胞系在体外暴露于甲氨蝶呤（MTX）会导致细胞体积呈剂量相关增加，同时细胞增殖减少。通过DT-5、11028和11028 + FeLV的半数抑制剂量（ID50）MTX浓度测量，这些细胞系对MTX的相对敏感性分别为118 nM、122 nM和28 nM。添加到用氚标记胸腺嘧啶脉冲标记的淋巴瘤细胞培养物中的ID50 MTX浓度的细胞动力学效应是TC延长约2小时，这归因于TS延长，而其他细胞周期阶段没有明显改变。当将这些细胞系接种10⁴个细胞移植到免疫抑制仓鼠的颊囊中时，它们具有高度致瘤性，会产生快速生长、血管丰富的肿瘤。
