Sibirnyĭ A A, Shavlovskiĭ G M, Goloshchapova G V
Genetika. 1977;13(5):872-9.
Riboflavin deficient mutant Pichia guilliermondii MS1 which requires approximately 1000-fold lower concentration of exogenous vitamin B2 for growth when compared with a non-adapted riboflavin deficient mutants of this species was isolated by means of of UV-irradiation. The growth of the mutant was strongly inhibited by actinomycin D and L-canavanine. The revertant MS8 and MS14 which synthesized riboflavin were selected from the strain MS1. These revertants posses a multiple sensitivity to actinomycin D, rifamycin, euflavine, mitomycin C, antimycin A, 8-azaadenine, 8-azaguanine, L-canavanine and 7-methyl-8-trifluoromethyl-10-(1'-D-ribityl)isoalloxazine. The ability to utilized glycerol and ethanol as a sole carbon source for growth was impaired in these mutants. The mutants which can utilize glycerol were isolated from the strain MS14. Such mutants were resistant to actonomycin D. Mutation (s) which determines a multiple sensitivity and inability to utilized glycerol was recessive.
通过紫外线照射分离出核黄素缺陷型突变体季也蒙毕赤酵母MS1，与该物种未适应的核黄素缺陷型突变体相比，其生长所需的外源维生素B2浓度大约低1000倍。突变体的生长受到放线菌素D和L-刀豆氨酸的强烈抑制。从菌株MS1中筛选出合成核黄素的回复突变体MS8和MS14。这些回复突变体对放线菌素D、利福霉素、优黄素、丝裂霉素C、抗霉素A、8-氮杂腺嘌呤、8-氮杂鸟嘌呤、L-刀豆氨酸和7-甲基-8-三氟甲基-10-(1'-D-核糖基)异咯嗪具有多重敏感性。这些突变体利用甘油和乙醇作为唯一碳源进行生长的能力受损。从菌株MS14中分离出能够利用甘油的突变体。此类突变体对放线菌素D具有抗性。决定多重敏感性和无法利用甘油的突变是隐性的。
