Hydrolysis of ribonucleoside 2',3'-cyclic phosphates by influenza and Newcastle disease viruses.

Two enzymatic activities hydrolysing ribonucleoside 2', 3'-cyclic phosphates (2', 3'-cNMP) to 2'- or 3'- nucleoside monophosphate were found associated with influenza and Newcastle disease viruses. The two enzymatic activities differed from each other by temperature optima and thermoresistance. 2', 3'-Cyclic nucleotide 3'-phosphohydrolase was responsible for splitting of the substrate to 2'-NMP. Splitting of the substrate to 3'-NMP was due either to ribonuclease or to 2', 3'-cyclic nucleotide 2'-phosphohydrolase.