Dartt D A, Torp-Pedersen C, Thorn N A
Brain Res. 1981 Jan 5;204(1):121-8. doi: 10.1016/0006-8993(81)90656-9.
In neurosecretosomes, isolated from ox neurohypophyses, both guanylate and adenylate cyclase activity was shown to be predominantly membrane-bound. Membrane-bound adenylate cyclase was inhibited by increasing the ionized calcium concentration from 10(-7) M to 10(-5) M, but was stimulated by calmodulin in the presence of 10(-7) M and 10(-5) M ionized calcium. In contrast, neither calcium ions nor calmodulin affected the activity of membrane-bound guanylate cyclase. Soluble cyclic AMP and cyclic GMP phosphodiesterase activities increased with increasing ionized calcium concentration (10(-7) M to 10(-3) M). At 10(-7) M ionized calcium concentration, both soluble phosphodiesterase activities were stimulated by calmodulin. Both the membrane-bound phosphodiesterase activities were inhibited by a high ionized calcium concentration (10(-3) M) and not affected by calmodulin.
从牛神经垂体中分离出的神经分泌小体中，鸟苷酸环化酶和腺苷酸环化酶活性主要都与膜结合。当离子钙浓度从10⁻⁷M增加到10⁻⁵M时，膜结合的腺苷酸环化酶受到抑制，但在存在10⁻⁷M和10⁻⁵M离子钙的情况下，它受到钙调蛋白的刺激。相比之下，钙离子和钙调蛋白都不影响膜结合鸟苷酸环化酶的活性。可溶性环磷酸腺苷和环磷酸鸟苷磷酸二酯酶活性随离子钙浓度增加（从10⁻⁷M到10⁻³M）而增加。在10⁻⁷M离子钙浓度下，两种可溶性磷酸二酯酶活性都受到钙调蛋白的刺激。两种膜结合磷酸二酯酶活性都受到高离子钙浓度（10⁻³M）的抑制，且不受钙调蛋白影响。
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