Miller L L
Diabetes. 1976;25(2 SUPPL):865-71.
The isolated rat liver perfused for 12 hours at pH 7.10 with a suspension of bovine erythrocytes in Krebs-Ringer bicarbonate buffer containing 3 per cent bovine serum albumin has been used as a test system to study effects of glucagon and of dexamethasone in the presence and absence of insulin on net biosynthesis of rat serum albumin, fibrinogen, alpah1-acid glycoprotein, alpha2-(acute phase) globulin, and haptoglobin. Quantitative measurement of perfusate glucose, amino acid nitrogen, and urea affords a basis for determining net glucose and nitrogen balance in the perfusion system. Although the dose of dexamethasone (total 1.0 mug.) used was insufficient to induce synthesis of alpha2-acute phase globulin, net syntheses of albumin, fibrogen, alpha1-acid glycoprotein, and haptoglobin were increased. Glucagon given with dexamethasone depressed albumin and haptoglobin synthesis markedly, but not that of fibrinogen and alpha1-acid glycoprotein. Glucagon with dexamethasone markedly enhanced ureogenesis and glycogenolysis and elicited an exaggerated negative nitrogen balance. The unfavorable effects of glucagon on albumin and haptoglobin synthesis and on nitrogen balance were reversed by giving insulin simultaneously. It is emphasized that insulin is essential for positive nitrogen balance.
将大鼠肝脏分离出来，在pH 7.10条件下，用含有3%牛血清白蛋白的Krebs - Ringer碳酸氢盐缓冲液中的牛红细胞悬液灌注12小时，以此作为测试系统，研究胰高血糖素和地塞米松在有或无胰岛素存在的情况下，对大鼠血清白蛋白、纤维蛋白原、α1 - 酸性糖蛋白、α2 - （急性期）球蛋白和触珠蛋白净生物合成的影响。对灌注液中的葡萄糖、氨基酸氮和尿素进行定量测量，为确定灌注系统中的净葡萄糖和氮平衡提供了依据。尽管所用的地塞米松剂量（总量1.0微克）不足以诱导α2 - 急性期球蛋白的合成，但白蛋白、纤维蛋白原、α1 - 酸性糖蛋白和触珠蛋白的净合成量增加。与地塞米松一起给予的胰高血糖素显著抑制白蛋白和触珠蛋白的合成，但不抑制纤维蛋白原和α1 - 酸性糖蛋白的合成。胰高血糖素与地塞米松一起显著增强尿素生成和糖原分解，并引发过度的负氮平衡。同时给予胰岛素可逆转胰高血糖素对白蛋白和触珠蛋白合成以及对氮平衡的不利影响。强调胰岛素对于正氮平衡至关重要。
