Pietrzak S M, Saz H J
Mol Biochem Parasitol. 1981 May;3(1):61-70. doi: 10.1016/0166-6851(81)90078-5.
The trematode, Fasciola hepatica, and the cestode, Spirometra mansonoides have been shown to be similar to the nematode Ascaris lumbricoides in that all three decarboxylate succinate to propionate plus CO2. Associated with this decarboxylation is an incorporation of 32Pi into organic phosphate. Both the decarboxylation and phosphorylation are markedly stimulated by the addition of propionyl-CoA, are dependent on coenzyme B12 and are inhibited by avidin. The trematode and cestode exhibit propionyl-CoA carboxylase, methylmalonyl-CoA mutase and acyl-CoA transferase activities in sonicated mitochondrial preparations. Data are consistent with the occurrence of a mitochondrial substrate level site for ATP generation which is coupled with the decarboxylation of succinate. In Fasciola preparations, acetyl-CoA stimulates the decarboxylation and phosphorylation to a considerably larger extent than propionyl-CoA, indicating the possibility that acetyl-CoA may serve physiologically in these reactions by donating the CoA moiety to succinate.
三者都将琥珀酸脱羧生成丙酸和二氧化碳。与这种脱羧作用相关的是将32Pi掺入有机磷酸盐中。添加丙酰辅酶A可显著刺激脱羧作用和磷酸化作用，二者都依赖于辅酶B12且受抗生物素蛋白抑制。在超声处理的线粒体制剂中，吸虫和绦虫表现出丙酰辅酶A羧化酶、甲基丙二酰辅酶A变位酶和酰基辅酶A转移酶活性。数据表明存在一个与琥珀酸脱羧作用相偶联的线粒体底物水平ATP生成位点。在肝片吸虫制剂中，乙酰辅酶A比丙酰辅酶A对脱羧作用和磷酸化作用的刺激程度大得多，这表明乙酰辅酶A可能通过将辅酶A部分提供给琥珀酸而在这些反应中发挥生理作用。
