用不同的仓鼠抗SV40-T血清检测人脑膜瘤中与SV40相关的肿瘤抗原

Demonstration of SV40-related tumour antigen in human meningiomas by different hamster SV40-T-antisera.

作者信息

Zimmermann W, Schernick S, Geissler E, Nisch G

出版信息

Acta Virol. 1981 Jul;25(4):199-204.

Abstract

Three of 5 human meningiomas tested in cell culture expressed SV40-related tumour (T) antigen as measured by indirect immunofluorescence with four different hamster anti-SV40 tumour sera. The per cent of T-antigen-positive nuclei varied depending on the antisera used. In one case (meningioma G. S.), only 25% of the T-antigen-positive nuclei detected with one antiserum could be demonstrated with another serum. The possible explanations are discussed. None of the meningioma cell cultures tested expressed SV40-related viral capsid (V) antigen.

摘要

在细胞培养中检测的5例人类脑膜瘤中，有3例通过用四种不同的仓鼠抗SV40肿瘤血清进行间接免疫荧光检测，表达了SV40相关肿瘤（T）抗原。T抗原阳性细胞核的百分比因所用抗血清而异。在一个病例（脑膜瘤G.S.）中，用一种抗血清检测到的T抗原阳性细胞核中，只有25%能用另一种血清证实。讨论了可能的解释。所检测的脑膜瘤细胞培养物均未表达SV40相关病毒衣壳（V）抗原。