生长抑素和胃泌素在大鼠体内释放至胃腔。

Somatostatin and gastrin release into the gastric lumen in rats.

作者信息

Koop H, Simson G, McIntosh C, Bedell R A, Arnold R, Creutzfeldt W

出版信息

Can J Physiol Pharmacol. 1981 Jun;59(6):574-9. doi: 10.1139/y81-086.

Abstract

Somatostatin and gastrin release into the gastric lumen was investigated in anaesthetized, vagally intact rats. The stomach was perfused at a flow rate of 0.5 mL.min-1. During perfusion with 0.1 M HCl or buffers of varying pH the somatostatin ans gastrin concentrations in the perfusate were less than 10 pg.mL -1 and approximately 30 pg.mL-1, respectively. Peptone caused a gastrin concentrations in the perfusate were less than 10 pg.mL-1 and approximately 30 pg.mL-1, respectively. Peptone caused a slight pH-independent increase in somatostatin release; gastrin release was unchanged despite an increase in serum gastrin from a basal of 15 +/- 4 to 155 +/- 34 pg.mL-1 during peptone stimulation. intravenous infusion of carbachol (1 microgram.kg-1.min-1) strongly stimulated luminal somatostatin and gastrin release (from 5 +/- 1 to 192 +/- 52 pg.mL-1 and from 27 +/- 5 to 198 +/- 41 pg.mL-1, respectively) during perfusion with 0.1 M HCl. Phosphate buffer perfusion at pH 7.5 abolished the cholinergic-mediated somatostatin release but the gastrin response was unaffected. It is suggested that changes of luminal hormone concentrations in the rat stomach do not reflect the secretory activity of the endocrine cells in the gastric mucosa.

摘要

在麻醉的、迷走神经完整的大鼠中研究了生长抑素和胃泌素释放到胃腔的情况。胃以0.5 mL·min⁻¹的流速进行灌注。在用0.1 M盐酸或不同pH值的缓冲液灌注期间，灌流液中生长抑素和胃泌素的浓度分别小于10 pg·mL⁻¹和约30 pg·mL⁻¹。蛋白胨引起生长抑素释放略有增加，且与pH无关；尽管在蛋白胨刺激期间血清胃泌素从基础值15±4 pg·mL⁻¹增加到155±34 pg·mL⁻¹，但胃泌素释放未发生变化。在灌注0.1 M盐酸期间，静脉输注卡巴胆碱（1 μg·kg⁻¹·min⁻¹）强烈刺激腔内生长抑素和胃泌素释放（分别从5±1 pg·mL⁻¹增加到192±52 pg·mL⁻¹和从27±5 pg·mL⁻¹增加到198±41 pg·mL⁻¹）。在pH 为7.5的磷酸盐缓冲液灌注时，胆碱能介导的生长抑素释放被消除，但胃泌素反应未受影响。提示大鼠胃腔内激素浓度的变化并不反映胃黏膜内分泌细胞的分泌活性。