Ngu J L, Neba G A, Leke N, Titanji V, Asonganyi T, Ndumbe P
Acta Trop. 1981 Sep;38(3):261-6.
A method for the selective recovery of living microfilariae from Onchocerca volvulus nodules is described. The microfilariae migrate through solidified agar gel into overlayering Hanks balanced salt solution (HBSS). The highest recovery rates of the worms were obtained with 0.3 and 0.4% agar. Optimal conditions for in vitro cultures of the larvae in HBSS were established; pH range 7.0-7.5, glucose concentration 2-5 mg/ml for long term cultures, osmolality 200-309 mOsmoles/l, temperature 4 degrees C for prolonged cultures and 24-28 degrees C for overall best yield of excretory/secretory products (ESP). Subculturing of the larvae reduced contamination of ESP with human serum protein to minimal amounts after 9 recultures done within 96 h.
本文描述了一种从盘尾丝虫结节中选择性回收活微丝蚴的方法。微丝蚴穿过凝固的琼脂凝胶迁移到上层覆盖的汉克斯平衡盐溶液（HBSS）中。使用0.3%和0.4%的琼脂可获得最高的蠕虫回收率。确定了幼虫在HBSS中体外培养的最佳条件；长期培养的pH范围为7.0 - 7.5，葡萄糖浓度为2 - 5mg/ml，渗透压为200 - 309毫渗透压/升，长时间培养的温度为4℃，排泄/分泌产物（ESP）总体产量最佳的温度为24 - 28℃。在96小时内进行9次再培养后，幼虫的传代培养将ESP中人类血清蛋白的污染降至最低水平。
