Tonoue T
Regul Pept. 1982 Jan;3(1):29-39. doi: 10.1016/0167-0115(82)90004-0.
An intracerebroventricular (i.c.v.) injection of TRH to the urethane anesthetized rat stimulates the activity of the superior laryngeal nerve (n.sl) which is a vagal ramus terminating at the thyroid gland and adjacent muscles. The response to TRH, a tonic increase in the n.sl outflow, was dose dependent in the 0.005-5.0 micrograms/100 g B.W. range. In contrast to this, methionine-enkephalin (ENK), neurotensin (NT) and somatostatin (SRIF) (5 micrograms/100 g, i.c.v.) all caused a transient decrease in n.sl activity. SRIF showed the highest attenuating effect when injected alone and was capable of diminishing the increased activity produced by a prior injection of TRH. ENK and NT failed to affect the TRH-induced increased activity. When injected concomitantly with TRH, SRIF blocked the response to TRH while ENK and NT both failed to affect the response to TRH. Pretreatment with triiodothyronine for 5 days strongly inhibited the response of the n.sl outflow to TRH. On the other hand, pretreatment with atropine, haloperidol, propranolol, phenoxybenzamine and p-chlorophenylalanine failed to block the stimulating effect of TRH although the response was diminished by some antagonists. It therefore seemed that TRH transmission is involved in central stimulation and SRIF is antagonistic in this regulation of n.sl outflow to the thyroid gland.
向经乌拉坦麻醉的大鼠脑室内注射促甲状腺激素释放激素（TRH），可刺激喉上神经（n.sl）的活动，该神经是迷走神经分支，终止于甲状腺及邻近肌肉。在0.005 - 5.0微克/100克体重范围内，对TRH的反应，即n.sl传出冲动的持续性增加呈剂量依赖性。与此相反，甲硫氨酸脑啡肽（ENK）、神经降压素（NT）和生长抑素（SRIF）（5微克/100克，脑室内注射）均导致n.sl活动短暂降低。单独注射时，SRIF的衰减作用最强，且能够减弱先前注射TRH所产生的活动增加。ENK和NT未能影响TRH诱导的活动增加。与TRH同时注射时，SRIF阻断了对TRH的反应，而ENK和NT均未能影响对TRH的反应。用三碘甲状腺原氨酸预处理5天可强烈抑制n.sl传出冲动对TRH的反应。另一方面，用阿托品、氟哌啶醇、普萘洛尔、酚苄明和对氯苯丙氨酸预处理未能阻断TRH的刺激作用，尽管某些拮抗剂使反应减弱。因此，似乎TRH传递参与中枢刺激，且SRIF在对甲状腺的n.sl传出调节中起拮抗作用。
