Walkenbach R J, LeGrand R D
Invest Ophthalmol Vis Sci. 1982 Jan;22(1):120-4.
Adenylate cyclase activity was measured in particulate fractions prepared from bovine and human corneal endothelium. Bovine enzyme activity was stimulated by fluoride, 5'-guanylylimidodiphosphate (GppNp) and beta-adrenergic agonists. Drug stimulation patterns of the human enzyme appeared qualitatively similar, but activities were generally higher than those of the bovine enzyme. This study demonstrates the existence of a receptor-mediated adenylate cyclase system in bovine and human corneal endothelium and suggests a possible role for cycli AMP in corneal endothelial physiology.
在从牛和人角膜内皮制备的微粒部分中测量了腺苷酸环化酶活性。牛酶活性受到氟化物、5'-鸟苷酰亚胺二磷酸（GppNp）和β-肾上腺素能激动剂的刺激。人酶的药物刺激模式在质量上似乎相似，但活性通常高于牛酶。本研究证明了牛和人角膜内皮中存在受体介导的腺苷酸环化酶系统，并提示环磷酸腺苷在角膜内皮生理学中可能发挥作用。
