Flickinger C J
J Cell Sci. 1976 Jul;21(2):291-302. doi: 10.1242/jcs.21.2.291.
Amoeba nuclear envelopes were damaged using microsurgery, and metabolic requirements for the steps in their repair were studied, and my placing the cells in a solution containing one of several metabolic inhibitors. The first step in repair, the association of pieces of endoplasmic reticulum with holes in the nuclear membranes, appears to be a passive process since it was not affected by inhibitors of energy production, RNA synthesis, or protein synthesis. In contrast, fusion of pieces of endoplasmic reticulum with the nuclear membranes at the margins of the holes was blocked by KCN and dinitrophenol, indicating that membrane fusion requires energy derived from respiration, but RNA and protein synthesis inhibitors did not prevent fusion of pieces of endoplasmic reticulum with the nuclear membranes. The subsequent completion of repair and restoration of intact nuclear membranes was almost completely blocked by inhibitors of respiration, and it was reduced in the presence of actinomycin and emetine, suggesting that in addition to a requirement for energy, some later steps in the repair of the nuclear membranes require RNA and protein synthesis.
利用显微手术破坏变形虫的核膜，并通过将细胞置于含有几种代谢抑制剂之一的溶液中，研究其修复步骤的代谢需求。修复的第一步，即内质网碎片与核膜上的孔的结合，似乎是一个被动过程，因为它不受能量产生、RNA合成或蛋白质合成抑制剂的影响。相比之下，内质网碎片在孔边缘与核膜的融合被氰化钾和二硝基苯酚阻断，这表明膜融合需要呼吸产生的能量，但RNA和蛋白质合成抑制剂并不能阻止内质网碎片与核膜的融合。随后修复的完成以及完整核膜的恢复几乎完全被呼吸抑制剂阻断，并且在放线菌素和吐根碱存在的情况下有所减少，这表明除了需要能量外，核膜修复的一些后续步骤还需要RNA和蛋白质合成。
