Kinne-Saffran E, Beauwens R, Kinne R
J Membr Biol. 1982;64(1-2):67-76. doi: 10.1007/BF01870769.
The rate of ATP hydrolysis in ATP-preloaded plasma membrane vesicles derived from the luminal membrane of renal cortical tubules, and the rate of H+ secretion out of the same vesicles were investigated. Both were inhibited at low temperature, by the action of filipin, an antibiotic that complexes with cholesterol in plasma membranes, and by the action of blockers of mitochondrial Fo hydrogen channels, dicyclohexylcarbodiimide and Dio-9. Valinomycin in the presence of K+ showed a stimulatory effect, the protonophor carbonyl-cyanid-p-trifluormethoxy-phenylhydrazone stimulated the intravesicular ATP hydrolysis and apparently abolished acidification of the extravesicular medium. Lowering of the pH of the extravesicular medium retarded ATP hydrolysis, while readjustment of extra- and intravesicular pH accelerated ATP hydrolysis again. These findings strongly support the assumption that an ATP-driven proton pump is located in the luminal membrane of renal cortical tubules.
研究了来源于肾皮质小管管腔膜的预加载ATP的质膜囊泡中的ATP水解速率，以及同一囊泡中H⁺的分泌速率。二者在低温下、在制霉菌素（一种与质膜中的胆固醇结合的抗生素）作用下以及在线粒体F₀氢通道阻滞剂二环己基碳二亚胺和Dio - 9作用下均受到抑制。在有K⁺存在的情况下缬氨霉素显示出刺激作用，质子载体羰基氰化物 - p - 三氟甲氧基 - 苯基腙刺激囊泡内ATP水解并明显消除囊泡外介质的酸化。降低囊泡外介质的pH会延迟ATP水解，而重新调节囊泡外和囊泡内的pH会再次加速ATP水解。这些发现有力地支持了肾皮质小管管腔膜中存在ATP驱动质子泵的假设。
