采用高效液相色谱法分析罗曼诺夫斯基血渍。
The analysis of Romanowsky blood stains by high-performance liquid chromatography.
作者信息
Dean W W, Lubrano G J, Heinsohn H G, Stastny M
出版信息
J Chromatogr. 1976 Sep 15;124(2):287-301. doi: 10.1016/s0021-9673(00)89744-7.
A high-performance liquid chromatographic procedure has been developed for separating and quantitating the components of thiazine dyes and compound blood stains. In terms of speed, quantitation, and component resolution, the assay reported here is superior to other reported chromatographic methods of cationic dye separation. The components present in commercial samples of thiazine dyes and LARCTM stain, a modified Wright-Giemsa stain, have been resolved in 25-40 min on a 5-mum microparticulate silica column using a methanol-water-glycine/acetic acid mobile phase. The mechanism of separation is demonstrated to be based predominantly upon the weak ion-exchange properties of silica.
已开发出一种高效液相色谱法，用于分离和定量噻嗪染料及复合血迹的成分。就速度、定量和成分分离度而言，本文报道的分析方法优于其他已报道的阳离子染料分离色谱方法。使用甲醇 - 水 - 甘氨酸/乙酸流动相，在5μm微粒硅胶柱上，噻嗪染料商业样品和改良瑞氏 - 吉姆萨染色剂LARCTM污渍中的成分在25 - 40分钟内得到分离。分离机制证明主要基于硅胶的弱离子交换特性。
Zotero 插件