Romagnano M A, Pilcher W H, Bennett-Clarke C, Chafel T L, Joseph S A
Brain Res. 1982 Feb 25;234(2):387-98. doi: 10.1016/0006-8993(82)90878-2.
Immunocytochemical analysis using antisera generated against the brain peptide somatostatin (SRIF) was examined in the brain of normal mice and in mice with chemical lesions of the arcuate nucleus produced neonatally by the administration of monosodium glutamate (MSG). In the normal mouse brain, SRIF immunoreactivity was seen in perikarya of the preoptic and hypothalamic periventricular nuclei. The normal distribution of SRIF fibers was apparent in several hypothalamic nuclei including the arcuate nucleus and in the internal and external zones of the median eminence. Extrahypothalamic sites of SRIF immunoreactive neurons and fibers were also observed throughout the telencephalon. At 60 days of age, certain neuroendocrine deficiencies, including growth parameters and obesity, were apparent in MSG-treated newborn mice. Analysis of SRIF projections in the brain of MSG-treated mice demonstrated a neurotoxic effect on arcuate neurons and a loss of SRIF projections to this region as well. Other components of the SRIF system in brain appeared unaffected. SRIF fibers of the arcuate region seem to originate from neuronal perikarya of the periventricular nucleus suggesting that MSG-induced endocrine deficiencies may be due to SRIF interactions at the level of the arcuate nucleus.
利用针对脑肽生长抑素（SRIF）产生的抗血清进行免疫细胞化学分析，对正常小鼠以及新生期经谷氨酸单钠（MSG）处理导致弓状核化学损伤的小鼠脑进行了检测。在正常小鼠脑中，视前核和下丘脑室周核的神经元胞体可见SRIF免疫反应性。SRIF纤维的正常分布在包括弓状核在内的几个下丘脑核以及正中隆起的内、外区很明显。在整个端脑也观察到了SRIF免疫反应性神经元和纤维的下丘脑外位点。在60日龄时，经MSG处理的新生小鼠出现了某些神经内分泌缺陷，包括生长参数和肥胖。对经MSG处理的小鼠脑内SRIF投射的分析表明，对弓状神经元有神经毒性作用，并且该区域的SRIF投射也丧失。脑内SRIF系统的其他成分似乎未受影响。弓状区域的SRIF纤维似乎起源于室周核的神经元胞体，这表明MSG诱导的内分泌缺陷可能是由于弓状核水平的SRIF相互作用所致。
