多种去极化剂对大鼠脑片血管活性肠肽释放的影响
Release of vasoactive intestinal peptide from rat brain slices by various depolarizing agents.
作者信息
Besson J, Rotsztejn W, Poussin B, Lhiaubet A M, Rosselin G
出版信息
Neurosci Lett. 1982 Mar 5;28(3):281-5. doi: 10.1016/0304-3940(82)90071-4.
The release of vasoactive intestinal peptide (VIP) from rat brain cortical and amygdala slices was studied by using various depolarizing agents such as potassium (K+), veratridine (VER) and batrachotoxin (BTX). The basal release of VIP observed is of the same order of magnitude for both structures and represents less than 0.1% of the tissue content per minute measured by a specific radioimmunoassay. Maximal stimulation obtained with 56 mMK+, 50 microM VER and 1 microM BTX corresponds to a mean 3-fold increase above the basal release of VIP in both cortex and amygdala. When the incubation medium did not contain any calcium, the action of potassium on the release of VIP was suppressed. When tetrodotoxin (1 microM) was added to the incubation medium, the veratridine- and batrachotoxin-induced release of VIP was inhibited whereas K+-induced release was unaffected. These results support the hypothesis that VIP can be a neurotransmitter in the central nervous system.
通过使用各种去极化剂，如钾（K +）、藜芦碱（VER）和蛙毒素（BTX），研究了大鼠脑皮质和杏仁核切片中血管活性肠肽（VIP）的释放。观察到的VIP基础释放量在这两个结构中处于相同的数量级，通过特定放射免疫测定法测得，其每分钟释放量不到组织含量的0.1%。用56 mM K +、50 μM VER和1 μM BTX获得的最大刺激对应于皮质和杏仁核中VIP基础释放量平均增加3倍。当孵育培养基中不含任何钙时，钾对VIP释放的作用受到抑制。当向孵育培养基中加入河豚毒素（1 μM）时，藜芦碱和蛙毒素诱导的VIP释放受到抑制，而K +诱导的释放不受影响。这些结果支持了VIP可能是中枢神经系统中神经递质的假说。
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