体外大鼠神经系统释放的免疫反应性生长抑素的特性研究
Characterization of immunoreactive somatostatin released from rat nervous system in vitro.
作者信息
Sheppard M C, Hendricks S, Hudson A, Kronheim S R
出版信息
Biochem J. 1982 Feb 1;201(2):321-8. doi: 10.1042/bj2010321.
There is considerable evidence that somatostatin is released from nerve terminals throughout the central nervous system in response to presynaptic stimulation, thus suggesting a neuromodulator role for the peptide. We here report the partial characterization of immunoreactive somatostatin released from rat nervous system in vitro (hypothalamus, spinal cord and hypothalamic, cortical, thalamic and striatal synaptosomes). Serial dilutions of released somatostatin immunoreactivity showed parallelism with dilutions of synthetic somatostatin standard. Somatostatin immunoreactivity released from all tissue areas coeluted with synthetic tetradecapeptide on Sephadex G-25 (fine grade) gel chromatography; more than 85% of this immunoreactivity bound to Sepharose-anti-somatostatin-serum immunoaffinity columns. In addition, immunoreactive material released from hypothalamus, spinal cord and hypothalamic and cortical synaptosomes inhibited somatotropin (growth hormone, 'STH', 'GH') release from perifused anterior pituitary in a dose-related manner, indicating biological similarity to synthetic somatostatin.
有大量证据表明，生长抑素在整个中枢神经系统中，会因突触前刺激而从神经末梢释放，这表明该肽具有神经调节作用。我们在此报告了体外从大鼠神经系统（下丘脑、脊髓以及下丘脑、皮质、丘脑和纹状体突触体）释放的免疫反应性生长抑素的部分特性。释放的生长抑素免疫反应性的系列稀释液与合成生长抑素标准品的稀释液呈平行关系。从所有组织区域释放的生长抑素免疫反应性，在Sephadex G - 25（细粒度）凝胶色谱上与合成十四肽共洗脱；超过85%的这种免疫反应性与琼脂糖 - 抗生长抑素 - 血清免疫亲和柱结合。此外，从下丘脑、脊髓以及下丘脑和皮质突触体释放的免疫反应性物质，以剂量相关的方式抑制了从灌流的垂体前叶释放的促生长素（生长激素，“STH”，“GH”），这表明其与合成生长抑素具有生物学相似性。
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