大鼠视网膜中具有免疫反应性和生物活性的生长抑素样物质。
Immunoreactive and biologically active somatostatin-like material in rat retina.
作者信息
Rorstad O P, Brownstein M J, Martin J B
出版信息
Proc Natl Acad Sci U S A. 1979 Jun;76(6):3019-23. doi: 10.1073/pnas.76.6.3019.
Somatostatin-like activity, as determined by radioimmunoassay and bioassay, is present in HCl extracts of rat retina. The concentrations of immunoreactive somatostatin are 612 +/- 43 (mean +/- SEM)pg per whole retina of 0.621 +/- 0.044 pg/microgram of protein in retinas from rats killed by decapitation, values which are not significantly different from those in retinas from rats killed by ether inhalation. The immunoreactive somatostatin was partially purified and concentrated by immunoaffinity chromatography. Both the crude retinal extracts and the immunoaffinity-purified immunoreactive somatostatin inhibited, in a dose-related manner, the release of rat growth hormone from dispersed rat anterior pituitary cells in culture. When the immunoaffinity-purified immunoreactive somotostatin was subjected to gel filtration chromatography, 96% of the recovered somatostatin eluted as a peak corresponding in position to that of synthetic somatostatin. Retinas from a group of rats with hereditary degeneration of the photoreceptor cells and another group of rats studied 1 year after transection of the optic nerves demonstrated an increased concentration of immunoreactive somatostatin compared to controls.
通过放射免疫测定法和生物测定法测定发现，大鼠视网膜的盐酸提取物中存在类生长抑素活性。断头处死大鼠的视网膜中，免疫反应性生长抑素的浓度为每只全视网膜612±43（平均值±标准误）皮克，蛋白质含量为0.621±0.044皮克/微克，这些值与乙醚吸入处死大鼠的视网膜中的值无显著差异。免疫反应性生长抑素通过免疫亲和色谱法进行部分纯化和浓缩。粗制视网膜提取物和免疫亲和纯化的免疫反应性生长抑素均以剂量相关的方式抑制培养的分散大鼠垂体前叶细胞释放大鼠生长激素。当对免疫亲和纯化的免疫反应性生长抑素进行凝胶过滤色谱分析时，96%回收的生长抑素以与合成生长抑素位置相对应的峰形式洗脱。与对照组相比，一组患有光感受器细胞遗传性退化的大鼠和另一组在视神经横断后1年研究的大鼠的视网膜显示免疫反应性生长抑素浓度增加。
Zotero 插件