Gartner L P, Alfonso R B, Provenza D V
Acta Histochem. 1982;70(1):89-98. doi: 10.1016/s0065-1281(82)80101-3.
Thiamine pyrophosphatase (TPPase), an enzyme associated with the Golgi apparatus, has been implicated in the regulation of cellular oxidation as well as of transport across cell membranes. This enzyme has been localized in odontogenic tissues of the postnatal mouse and it was the intent of the present study to localize TPPase during prenatal odontogenesis. Mouse fetuses (CDI, Charles River) 14 through 19 days postconception were decapitated, the heads were frozen and mounted on the chuck of a cryostat. Frontal sections, 14 micrometers thick, were air-dried and incubated for TPPase activity. Subsequent to incubation the activity was visualized by immersion in 1% ammonium sulfide. The degree of enzyme activity varied not only with the chronological age of the fetus but also as a function of the tissue's metabolic state. Regions, such as the dental lamina, evidenced decreased TPPase activity with increasing age, while tissue layers such as the IEE displayed greater enzyme activity with increasing age.
硫胺素焦磷酸酶（TPPase）是一种与高尔基体相关的酶，它参与细胞氧化调节以及跨细胞膜运输的调节。这种酶已定位在出生后小鼠的牙源性组织中，本研究旨在确定产前牙胚发生过程中TPPase的定位。将妊娠14至19天的小鼠胎儿（CDI，Charles River）断头，头部冷冻后固定在低温恒温器的卡盘上。切取14微米厚的额状切片，空气干燥后进行TPPase活性孵育。孵育后，通过浸入1%硫化铵中使活性可视化。酶活性程度不仅随胎儿的年龄而变化，还与组织的代谢状态有关。随着年龄增长，牙板等区域的TPPase活性降低，而内釉上皮（IEE）等组织层的酶活性则随着年龄增长而增强。
