Ishikawa K, Martinez J L, McCaugh J L
J Chromatogr. 1982 Sep 10;231(2):255-64. doi: 10.1016/s0378-4347(00)81850-2.
A simple procedure for the simultaneous determination of morphine and monoamine transmitters was developed. The procedure consisted of (1) n-butanol extraction and (2) separation and quantitative determination by means of high-performance liquid chromatography combined with electrochemical detection. The maximum intracerebral concentration (210 +/- 35 ng/g wet tissue) of morphine was detected 30 min after intramuscular injection (10 mg/kg), which agreed with previous research. Noradrenaline was significantly decreased by morphine injection, while dopamine and 5-hydroxytryptamine were unchanged. However, 3-methoxytyramine, a metabolite of dopamine, was increased, suggesting that the drug increased the turnover rate of dopamine. The procedure used revealed a direct correlation between pharmacokinetics (e.g., distribution of morphine) and pharmacodynamics (e.g. changes of monoamine concentrations) of the drug in vivo.
开发了一种同时测定吗啡和单胺递质的简单方法。该方法包括：（1）正丁醇萃取；（2）采用高效液相色谱结合电化学检测进行分离和定量测定。肌肉注射（10mg/kg）后30分钟检测到吗啡的最大脑内浓度为（210±35ng/g湿组织），这与先前的研究结果一致。注射吗啡后去甲肾上腺素显著降低，而多巴胺和5-羟色胺未发生变化。然而，多巴胺的代谢产物3-甲氧基酪胺增加，表明该药物增加了多巴胺的周转率。所采用的方法揭示了该药物在体内的药代动力学（如吗啡的分布）和药效学（如单胺浓度的变化）之间的直接相关性。
