非洲爪蟾卵母细胞中的一种钙依赖性瞬时外向电流。

A calcium-dependent transient outward current in Xenopus laevis oocytes.

作者信息

Miledi R

出版信息

Proc R Soc Lond B Biol Sci. 1982 Jul 22;215(1201):491-7. doi: 10.1098/rspb.1982.0056.

DOI:10.1098/rspb.1982.0056

Abstract

Membrane currents were investigated in Xenopus laevis oocytes under voltage clamp. Depolarizing pulses, given from a holding potential of about-100 mV, elicited a transient outward current when the membrane potential was made more positive than about-20 mV. As the potential was made increasingly positive the transient outward current first increased and then decreased. The amplitude of the transient current increased when the external Ca2+ concentration was raised; and the current was abolished by Mn2+. It appears that when the membrane is depolarized Ca2+ ions enter the oocyte and trigger an outward current, possibly by opening C1- channels.

摘要

在电压钳制条件下，对非洲爪蟾卵母细胞的膜电流进行了研究。从约 -100 mV 的钳制电位给出去极化脉冲，当膜电位变得比约 -20 mV 更正时，会引发一个短暂的外向电流。随着电位变得越来越正，短暂外向电流先增加然后减小。当外部 Ca2+ 浓度升高时，短暂电流的幅度增加；并且该电流被 Mn2+ 消除。似乎当膜去极化时，Ca2+ 离子进入卵母细胞并可能通过打开 Cl- 通道触发外向电流。