呼肠孤病毒突变体G组的基因组片段分配
Assignment of reovirus mutant group 'G' to its genomic segment.
作者信息
Hossain A
出版信息
Acta Virol. 1982 Sep;26(5):395-9.
PMID:6128908
Abstract
Temperature sensitive (ts+) recombinants between the UV-inactivated reovirus type 1 and the group G ts mutant of reovirus type 3 were obtained using a cross-reactivation technique. Analysis of the dsRNA of recombinant clones in PAGE allowed a preliminary mapping of the ts mutation into its genomic S3 segment. This was confirmed by the hybridization test.
摘要
利用交叉复活技术获得了紫外线灭活的呼肠孤病毒1型与呼肠孤病毒3型G组温度敏感（ts+）重组体。通过聚丙烯酰胺凝胶电泳（PAGE）对重组克隆的双链RNA进行分析，初步将ts突变定位到其基因组S3片段。杂交试验证实了这一点。
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