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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Hijazi A H, Chefurka W

Comp Biochem Physiol C Comp Pharmacol. 1982;73(2):369-75. doi: 10.1016/0306-4492(82)90137-x.

The interaction of mitochondrial and submitochondrial membranes with DDT, methoxychlor and kelthane as monitored by fluorescence changes of ANS was studied. 2. The three organochlorine pesticide chemicals reduced the succinate- and ATP-dependent quenching of ANS fluorescence of mitochondria in a concentration-dependent manner. The results suggest that they blocked the supply of energy to the inner membrane. 3. The reversal of the substrate-induced quenching of ANS by uncouplers was abolished by DDT and kelthane probably as a consequence of the inhibition of protonophoric activity of the uncoupler. 4. Both DDT and kelthane were relatively ineffective inhibitors of substrate-induced quenching of ANS fluorescence of submitochondrial particles. The results suggest an asymmetry of the inner membrane with respect to DDT-inhibition of substrate-induced energization. 5. The possible basis of this asymmetry as well as of inhibition of energy supply to the membrane is discussed.

研究了用ANS荧光变化监测的线粒体和亚线粒体膜与滴滴涕、甲氧滴滴涕和开乐散的相互作用。2. 这三种有机氯农药化学物质以浓度依赖的方式降低了线粒体中琥珀酸和ATP依赖的ANS荧光猝灭。结果表明它们阻断了内膜的能量供应。3. 解偶联剂对底物诱导的ANS猝灭的逆转被滴滴涕和开乐散消除，这可能是由于解偶联剂的质子载体活性受到抑制。4. 滴滴涕和开乐散对亚线粒体颗粒底物诱导的ANS荧光猝灭的抑制作用相对较弱。结果表明内膜在滴滴涕抑制底物诱导的能量化方面存在不对称性。5. 讨论了这种不对称性以及膜能量供应抑制的可能基础。

Hijazi A H, Chefurka W

Comp Biochem Physiol C Comp Pharmacol. 1982;73(2):369-75. doi: 10.1016/0306-4492(82)90137-x.

The interaction of mitochondrial and submitochondrial membranes with DDT, methoxychlor and kelthane as monitored by fluorescence changes of ANS was studied. 2. The three organochlorine pesticide chemicals reduced the succinate- and ATP-dependent quenching of ANS fluorescence of mitochondria in a concentration-dependent manner. The results suggest that they blocked the supply of energy to the inner membrane. 3. The reversal of the substrate-induced quenching of ANS by uncouplers was abolished by DDT and kelthane probably as a consequence of the inhibition of protonophoric activity of the uncoupler. 4. Both DDT and kelthane were relatively ineffective inhibitors of substrate-induced quenching of ANS fluorescence of submitochondrial particles. The results suggest an asymmetry of the inner membrane with respect to DDT-inhibition of substrate-induced energization. 5. The possible basis of this asymmetry as well as of inhibition of energy supply to the membrane is discussed.

研究了用ANS荧光变化监测的线粒体和亚线粒体膜与滴滴涕、甲氧滴滴涕和开乐散的相互作用。2. 这三种有机氯农药化学物质以浓度依赖的方式降低了线粒体中琥珀酸和ATP依赖的ANS荧光猝灭。结果表明它们阻断了内膜的能量供应。3. 解偶联剂对底物诱导的ANS猝灭的逆转被滴滴涕和开乐散消除，这可能是由于解偶联剂的质子载体活性受到抑制。4. 滴滴涕和开乐散对亚线粒体颗粒底物诱导的ANS荧光猝灭的抑制作用相对较弱。结果表明内膜在滴滴涕抑制底物诱导的能量化方面存在不对称性。5. 讨论了这种不对称性以及膜能量供应抑制的可能基础。