脑鸟苷酸环化酶在进化过程中的抗原保守性。

Antigenic conservation of brain guanylate cyclase during evolution.

作者信息

Basset P, Zwiller J, Ghandour M S, Revel M O, Thiébaut J B

出版信息

J Neuroimmunol. 1982 Dec;3(4):285-94. doi: 10.1016/0165-5728(82)90032-7.

Abstract

A comparative study of brain guanylate cyclase from different animal species (including man, bird, fish and amphibian) has been performed using a specific antibody directed against soluble rat brain guanylate cyclase. Analyses were performed on supernatant fractions by the double-immunodiffusion test, by the protein blotting technique after SDS-polyacrylamide gel electrophoresis and by analytical isoelectric focusing on agarose allowing specific immunodetection of isoelectric patterns. Membrane-bound guanylate cyclase from rat brain and soluble guanylate cyclase from several rat tissues cross-reacted with the antibody. All the brain enzymes tested were found to be identical by double-immunodiffusion. The electrophoretic and isoelectrophoretic profiles of the different brain guanylate cyclases were found to exhibit many common features with some differences between mammalian and non-mammalian enzymes. In human brain, guanylate cyclase has been localized in glial and neuronal cells by immunohistochemistry. The results demonstrate that guanylate cyclase has been well conserved during the course of evolution and are consistent with the involvement of guanylate cyclase and cyclic GMP in basic cellular function.

摘要

利用针对可溶性大鼠脑鸟苷酸环化酶的特异性抗体，对来自不同动物物种（包括人类、鸟类、鱼类和两栖动物）的脑鸟苷酸环化酶进行了比较研究。通过双向免疫扩散试验、SDS-聚丙烯酰胺凝胶电泳后的蛋白质印迹技术以及在琼脂糖上进行分析等电聚焦以实现等电模式的特异性免疫检测，对上清液组分进行了分析。大鼠脑的膜结合鸟苷酸环化酶和几种大鼠组织的可溶性鸟苷酸环化酶与该抗体发生交叉反应。通过双向免疫扩散发现，所有测试的脑酶都是相同的。不同脑鸟苷酸环化酶的电泳和等电泳图谱显示出许多共同特征，哺乳动物和非哺乳动物的酶之间存在一些差异。在人类大脑中，通过免疫组织化学方法已将鸟苷酸环化酶定位在神经胶质细胞和神经元细胞中。结果表明，鸟苷酸环化酶在进化过程中得到了很好的保留，这与鸟苷酸环化酶和环磷酸鸟苷参与基本细胞功能的情况一致。