Kawashima Y, Kozuka H
Biochim Biophys Acta. 1982 Dec 13;713(3):622-8.
Male rats were fed a diet containing 0.5% (w/w) p-chlorophenoxyisobutyric acid (clofibric acid), a hypolipidemic drug. Activities of stearoyl-CoA desaturation in hepatic microsomes were increased approx. 4 times following the administration of clofibric acid for 7 days. An increase in the activity of desaturation of stearic acid was also observed in the liver of clofibric acid-fed rats in vivo. The increase in the activity of microsomal stearoyl-CoA desaturation by clofibric acid-feeding was due to the increase in the activity of terminal desaturase as measured by the rate constant for cytochrome b5 reoxidation, but not due to the changes in cytochrome b5 content and NADH-cytochrome b5 reductase activity. Increases in the activity of stearoyl-CoA desaturation by clofibric acid-feeding were also observed in rats of hormonally altered state, such as diabetic rats, hyperthyroid rats and hypothyroid rats. Percentages of octadecenoic acid in total fatty acid of hepatic lipid were increased with the increase in the activity of stearoyl-CoA desaturation.
给雄性大鼠喂食含有0.5%（w/w）对氯苯氧异丁酸（氯贝酸）的饮食，氯贝酸是一种降血脂药物。服用氯贝酸7天后，肝微粒体中硬脂酰辅酶A去饱和酶的活性大约增加了4倍。在体内，喂食氯贝酸的大鼠肝脏中也观察到硬脂酸去饱和酶活性增加。喂食氯贝酸导致微粒体硬脂酰辅酶A去饱和酶活性增加，是由于通过细胞色素b5再氧化速率常数测量的末端去饱和酶活性增加，而不是由于细胞色素b5含量和NADH - 细胞色素b5还原酶活性的变化。在激素状态改变的大鼠，如糖尿病大鼠、甲状腺功能亢进大鼠和甲状腺功能减退大鼠中，也观察到喂食氯贝酸后硬脂酰辅酶A去饱和酶活性增加。随着硬脂酰辅酶A去饱和酶活性增加，肝脏脂质总脂肪酸中十八碳烯酸的百分比也增加。
