Kondo Y, Nagatsu I, Yoshida M, Karasawa N, Nagatsu T
Cell Tissue Res. 1983;228(2):405-8. doi: 10.1007/BF00204888.
The localization of catecholamine-synthesizing enzymes [tyrosine hydroxylase (TH), dopamine-beta-hydroxylase (DBH), and phenylethanolamine-N-methyltransferase (PNMT)], of serotonin (5-HT), and adrenocorticotropin (ACTH) in the pituitary of bullfrog (Rana catesbeiana), rat, hamster, and dog was examined by the immunofluorescence method. Many TH- and DBH-positive but PNMT-negative noradrenalin cells and 5-HT-positive serotonin cells were first observed in the pars distalis of the frog pituitary together with small numbers of ACTH-positive cells, in marked contrast to our previous findings that TH-positive but DBH-negative dopamine cells are rare in the anterior lobe of rat and dog pituitary. The entire population of cells of the pars intermedia showed a weak ACTH-like immunoreaction. Although most of these cells were TH- or 5-HT-negative, TH-positive but DBH-negative dopaminergic varicose fibers surrounded these cells. Among cells of the pars intermedia of the frog, 5-HT-positive cells with processes were also scattered. In the neural lobe, TH- and 5-HT-immunoreactive fibers were rarely seen.
采用免疫荧光法检测了牛蛙（牛蛙）、大鼠、仓鼠和狗垂体中儿茶酚胺合成酶[酪氨酸羟化酶（TH）、多巴胺-β-羟化酶（DBH）和苯乙醇胺-N-甲基转移酶（PNMT）]、5-羟色胺（5-HT）和促肾上腺皮质激素（ACTH）的定位。在蛙垂体远侧部首次观察到许多TH和DBH阳性但PNMT阴性的去甲肾上腺素细胞以及5-HT阳性的5-羟色胺细胞，同时还有少量ACTH阳性细胞，这与我们之前的发现形成显著对比，即在大鼠和狗垂体前叶中TH阳性但DBH阴性的多巴胺细胞很少见。垂体中间部的所有细胞均显示出较弱的ACTH样免疫反应。虽然这些细胞大多为TH或5-HT阴性，但TH阳性但DBH阴性的多巴胺能曲张纤维围绕着这些细胞。在蛙垂体中间部的细胞中，也散在有带突起的5-HT阳性细胞。在神经叶中，很少见到TH和5-HT免疫反应性纤维。
